Research
The Grant group uses genomic and functional genomic approaches, combined with advanced molecular, proteomic and microscopy techniques, and often integrated with mathematical and statistical models, to determine the basis by which bacterial pathogens survive in the environment, colonise their hosts and cause disease. The Grant group have been at the forefront of developing genomic and functional genomic approaches to study bacterial pathogens of clinical and veterinary significance, including transposon mutagenesis, Tn-Seq, TraDIS and CRISPR technologies. Additionally, the Grant group have developed Hi-C laboratory and bioinformatic pipelines for the study of complex microbiomes and for disambiguating plasmids withing bacterial populations, including tracking antimicrobial resistance genes in complex microbial communities.
Publications
de Vries SPW, Gupta S, Baig A, Wright E, Wedley A, Jensen AN, Lora LL, Humphrey S, Skovgard H, Macleod K, Pont E, Wolanska DP, L’Heureux J, Mobegi FM, Smith DGE, Everest P, Zomer A, Williams N, Wigley P, Humphrey T, Maskell DJ, Grant AJ. (2017) Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models. Sci. Rep. 7, 1251. (PMID: 28455506. PMCID: PMC5430854. DOI: 10.1038/s41598-107-01133-4).
Oshota O, Conway M, Fookes M, Schreiber F, Chaudhuri RR, Yu L, Morgan FJE, Clare S, Choudhary J, Thomson NR, Lio P, Maskell DJ, Mastroeni P, Grant AJ. (2017) Transcriptome and proteome analysis of Salmonella enterica serovar Typhimurium systemic infection of wild type and immune-deficient mice. PLoS One. 12(8): e0181365. (PMID: 28796790. PMCID: PMC5552096. DOI: 10.1371/journal.pone.0181365).
Esson D, Mather AE, Scanlan E, Gupta S, de Vries SP, Bailey D, Harris SR, McKInley TJ, Meric G, Berry SK, Mastroeni P, Sheppard SK, Christie G, Thomson NR, Parkhill J, Maskell DJ, Grant AJ. (2016) Genomic variations leading to alterations in cell morphology of Campylobacter spp. Sci. Rep. 6: 38303. (PMID: 27910897. PMCID: PMC5133587. DOI: 10.1038/srep38303).
Grant AJ, Morgan FJE, McKinley TJ, Foster GL, Maskell DJ, Mastroeni P. (2012) Attenuated Salmonella Typhimurium lacking the pathogenicity island-2 type 3 secretion system grow to high bacterial numbers inside phagocytes in mice. PLoS Pathog. 8(12): e1003070. (PMID: 23236281. PMCID: PMC3516571. DOI: 10.1371/journal.ppat.1003070).
Grant AJ, Restif O, McKinley TJ, Sheppard M, Maskell DJ, Mastroeni P. (2008) Modelling within-host spatiotemporal dynamics of invasive bacterial disease. PLoS Biol. 6(4), e74. (PMID: 18399718. PMCID: PMC2288627. DOI: 10.1371/journal.pbio.0060074).
